Rapid Detection of Multidrug-Resistant Mycobacterium tube rculosis by Use of Real-Time PCR and High-Resolution Melt Analysis

Detection and discrimination of two Brucella species by multiplex real-time PCR and high-resolution melt analysis curve from human blood and comparison of results using RFLP

Objective(s): Rapid and accurate detection of Brucella abortus and Brucella melitensis from clinical samples is so important because antibiotic treatment has major side effects. This study reveals a new method in detection of clinical samples of brucellosis using real-time PCR and high-resolution melt (HRM) curve analysis. Materials and Methods: 160 brucellosis suspicious samples with more tha...

Rapid detection of Multidrug Resistant Tuberculosis using real-time PCR and High Resolution Melt analysis Running title: Rapid detection of MDR-TB

Real-time PCR and high-resolution melt analysis for rapid detection of Mycobacterium leprae drug resistance mutations and strain types.

Drug resistance surveillance and strain typing of Mycobacterium leprae are necessary to investigate ongoing transmission of leprosy in regions of endemicity. To enable wider implementation of these molecular analyses, novel real-time PCR-high-resolution melt (RT-PCR-HRM) assays without allele-specific primers or probes and post-PCR sample handling were developed. For the detection of mutations ...

rapid detection of rifampicin- and isoniazid-resistant mycobacterium tuberculosis using real-time pcr

conclusions detection of drug resistance associated with mutations through real-time pcr by melting analysis technique showed a high differentiating power. this technique had high concordance with the standard proportion test and mas-pcr results. patients and methods in a cross-sectional study carried out in 2014, 90 patients with m. tuberculosis from five border provinces of iran were selected...

Rapid identification of multidrug-resistant Mycobacterium tuberculosis isolates by rpoB gene scanning using high-resolution melting curve PCR analysis.

BACKGROUND Multidrug-resistant (MDR) Mycobacterium tuberculosis poses a serious threat to the control of tuberculosis (TB) and constitutes an increasing public health problem. The availability of rapid in vitro susceptibility tests is a prerequisite for optimal patient treatment. Rifampicin resistance caused by diverse mutations in the rpoB gene is an established and widely used surrogate marke...